Anti-Bax Anticorps [2D2] (A253002)

Name: Anti-Bax Antibody [2D2] - BSA and Azide free
Brand: Antibodies.com
SKU: A253002
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [2D2] de souris contre Bax pour Flow Cytometry, IF, WB et IHC-P.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

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Spécifications

Nom du produit

Anti-Bax Antibody [2D2] - BSA and Azide free

Description du produit

Mouse monoclonal [2D2] antibody to Bax.

Spécificité

This antibody recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Applications

Flow Cytometry, IF, WB, IHC-P

Recommander des dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reactivité

Human, Monkey

Réactivité croisée

This antibody does not cross react with Mouse or Rat.

Immunogène

Synthetic peptide corresponding to amino acids 3-16 of human Bax protein.

Séquence

Cys-GSGEQPRGGGPTSS

Hôte

Mouse

Clonalité

Monoclonal

Clone

2D2

Isotype

IgG1

Chaînes légères

kappa

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Masse moléculaire

21 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Bax Antibody [2D2] (A249822).

Synonymes

Apoptosis regulator BAX, Bax-protein, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, BAX_HUMAN, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunohistochemistry - Anti-Bax Antibody [2D2] - BSA and Azide free (A253002)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma using Anti-Bax Antibody [2D2].

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Figure 2: Flow Cytometry - Anti-Bax Antibody [2D2] - BSA and Azide free (A253002)

Flow cytometric analysis of human Jurkat cells using Anti-Bax Antibody [2D2] followed by Goat Anti-Mouse IgG (CF® 488) (Orange). Unstained cells (Blue). Isotype Control (Red).

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Figure 3: Immunofluorescence - Anti-Bax Antibody [2D2] - BSA and Azide free (A253002)

Immunofluorescent analysis of human Jurkat cells; Panel A: Stained with Anti-Bax Antibody [2D2] (CF® 488) (Green); Panel B: Stained with Goat anti-Mouse IgG (CF® 488); Nuclear counterstain is RedDot.

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Figure 4: SDS-PAGE - Anti-Bax Antibody [2D2] - BSA and Azide free (A253002)

SDS-PAGE analysis of Anti-Bax Antibody [2D2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.