Anti-Bax Antibody [2D2] (A249822)

Anti-Bax Antibody [2D2]

Mouse monoclonal [2D2] antibody to Bax.

This antibody recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Monkey

This antibody does not cross react with Mouse or Rat.

Synthetic peptide corresponding to amino acids 3-16 of human Bax protein.

Cys-GSGEQPRGGGPTSS

Mouse

Monoclonal

2D2

IgG1

Protein A/G chromatography.

200 µg/ml

21 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Bax Antibody [2D2] - BSA and Azide free (A253002).

Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BCL2L4