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Anti-ZNRF1 Antibody (A82537)

This product is identical to Abcam (ab175125). Discover more for less.

Western Blot - Anti-ZNRF1 Antibody (A82537) Immunohistochemistry - Anti-ZNRF1 Antibody (A82537) Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)
$480
Product Datasheet

Goat polyclonal antibody to ZNRF1 for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-ZNRF1 Antibody
Description
Goat polyclonal antibody to ZNRF1.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:32,000, WB: 1-3µg/ml, IHC: 10µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the internal region of mouse ZNRF1.
Sequence
C-SDSTYAHGNGYQET
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
28 kDa
Predicted MW
23.8kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
B830022L21Rik, DKFZp434E229, E3 ubiquitin protein ligase ZNRF1, E3 ubiquitin-protein ligase ZNRF1, EC 6.3.2.-, FLJ14846, MGC101991, MGC15430, Nerve injury gene 283, Nerve injury induced gene 283 protein, Nerve injury-induced gene 283 protein, NIN283, Rnf42, Zinc and ring finger 1, zinc and ring finger protein 1, zinc finger and ring finger protein 1, Zinc/RING finger protein 1, ZNRF 1, ZNRF1_HUMAN, Zrfp1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ZNRF1 Antibody (A82537)Enlarge Image
Figure 1: Western Blot - Anti-ZNRF1 Antibody (A82537)
N2a expressing Mouse Znrf1 (mock transfection in first lane) and probed with Anti-ZNRF1 Antibody (A82537) (1µg/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)Enlarge Image
Figure 2: Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)
Anti-ZNRF1 Antibody (A82537) (10µg/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)Enlarge Image
Figure 3: Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)
Anti-ZNRF1 Antibody (A82537) (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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