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Anti-ZNRF1 Antibody (A82537)

Western Blot - Anti-ZNRF1 Antibody (A82537) - Antibodies.com Immunohistochemistry - Anti-ZNRF1 Antibody (A82537) - Antibodies.com Immunohistochemistry - Anti-ZNRF1 Antibody (A82537) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to ZNRF1 for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-ZNRF1 Antibody
Description
Goat polyclonal antibody to ZNRF1.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:32,000, WB: 1-3µg/ml, IHC: 10µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the internal region of mouse ZNRF1.
Sequence
C-SDSTYAHGNGYQET
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
28 kDa
Predicted MW
23.8kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
B830022L21Rik, DKFZp434E229, E3 ubiquitin protein ligase ZNRF1, E3 ubiquitin-protein ligase ZNRF1, EC 6.3.2.-, FLJ14846, MGC101991, MGC15430, Nerve injury gene 283, Nerve injury induced gene 283 protein, Nerve injury-induced gene 283 protein, NIN283, Rnf42, Zinc and ring finger 1, zinc and ring finger protein 1, zinc finger and ring finger protein 1, Zinc/RING finger protein 1, ZNRF 1, ZNRF1_HUMAN, Zrfp1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ZNRF1 Antibody (A82537) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ZNRF1 Antibody (A82537)
ZNRF1 expression in N2a cell lysate and Mouse Brain lysates (Embryo E14 and adult cerebellum) analyzed by western blot. Cells were transfected to overexpress ZNRF1 and incubated with Anti-ZNRF1 Antibody (A82537) at 1µg/ml and detcted by chemiluminescence.
Immunohistochemistry - Anti-ZNRF1 Antibody (A82537) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)
ZNRF1 expression in Mouse Cerebral Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-ZNRF1 Antibody (A82537) at 10µg/ml and revealed with a biotinylated Anti-goat secondary antibody and streptavidin-conjugated Alexa 488. Note the Neurofilament M was labeled by Millipore at a dilution of 1:100.
Immunohistochemistry - Anti-ZNRF1 Antibody (A82537) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-ZNRF1 Antibody (A82537)
ZNRF1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ZNRF1 Antibody (A82537) at 5µg/ml and revealed with alkaline phosphatase (AP).
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