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Anti-ZNF423 Antibody (A285990)

Immunofluorescence - Anti-ZNF423 Antibody (A285990) - Antibodies.com Flow Cytometry - Anti-ZNF423 Antibody (A285990) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to ZNF423 for ELISA, IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-ZNF423 Antibody
Description
Goat polyclonal antibody to ZNF423.
Applications
ELISA, IF, Flow Cytometry
Dilutions
ELISA: 1:2,000, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region (near N terminus) of human ZNF423.
Sequence
EPECDQKTSRALEDR
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Early B cell factor associated zinc finger protein, Ebfaz, hOAZ, JBTS19, KIAA0760, NPHP14, Nur12, OAZ, OLF 1/EBF associated zinc finger, OLF1/EBF associated zinc finger protein, Olf1/EBF-associated zinc finger protein, Roaz, Smad and Olf interacting zinc finger protein, Smad- and Olf-interacting zinc finger protein, Zfp104, ZFP423, Zinc finger protein 423, zinc finger protein 423, mouse, homolog of, ZN423_HUMAN, ZNF423 zinc finger protein 423
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-ZNF423 Antibody (A285990) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-ZNF423 Antibody (A285990)
ZNF423 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ZNF423 Antibody (A285990) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Flow Cytometry - Anti-ZNF423 Antibody (A285990) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-ZNF423 Antibody (A285990)
ZNF423 expression in Kelly cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ZNF423 Antibody (A285990) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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