Anti-ZAP70 Antibody [ZAP-03] (A85696)

Mouse monoclonal [ZAP-03] antibody to ZAP70 for WB, ICC and Flow Cytometry (Intracellular).

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-ZAP70 Antibody [ZAP-03]
Description
Mouse monoclonal [ZAP-03] antibody to ZAP70.
Specificity
This antibody reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Applications
WB, ICC, Flow Cytometry (Intracellular)
Dilutions
Flow Cytometry: Intracellular staining: 2-5 µg/ml; Positive Control: HPB-ALL human peripheral blood T cell leukemia cell line, WB: 0.5-2 µg/ml.
Reactivity
Human
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 protein with histidine tag.
Host
Mouse
Clonality
Monoclonal
Clone ID
ZAP-03
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
70 kDa zeta associated protein, 70 kDa zeta-associated protein, 70 KDa Zeta-Associated Protein2, 70 KDa Zeta-Chain Associated Protein, EC 2.7.10, EC 2.7.10.2, FLJ17670, FLJ17679, Selective T cell defect, SRK, STCD, STD, Syk related tyrosine kinase, Syk-related tyrosine kinase, Truncated ZAP kinase, Tyrosine protein kinase ZAP70, Tyrosine-protein kinase ZAP-70, TZK, ZAP 70, ZAP-70, ZAP70_HUMAN, Zeta chain associated protein kinase 70kD, Zeta chain associated protein kinase 70kDa, Zeta chain associated protein kinase 70kDa isoform 1, Zeta chain associated protein kinase 70kDa isoform 2, Zeta chain of T cell receptor associated protein kinase 70, Zeta chain TCR associated protein kinase 70kD, Zeta chain TCR associated protein kinase 70kDa, Zeta-Chain (TCR) Associated ProteinKinase (70 KD), Zeta-Chain Associated Protein Kinase, 70kD
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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