Anti-YY1 Antibody [ARC0048] (A308157)

Name: Anti-YY1 Antibody [ARC0048]
Brand: Antibodies.com
SKU: A308157
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0048] antibody to YY1 for WB, ICC/IF, IP and ChIP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Nuclear Loading Control

Specifications

Name

Anti-YY1 Antibody [ARC0048]

Description

Rabbit monoclonal [ARC0048] antibody to YY1.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000, ChIP: 1:1,000-1:5,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490).

Sequence

PGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0048

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CF1, Delta, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, NF-E1, NFE1, OTTHUMP00000197459, Transcriptional repressor protein YY1, TYY1_HUMAN, UCR motif DNA binding protein, UCRBP, Yin and yang 1, Yin and Yang 1 protein, Yin Yang 1, Ying Yang 1, YY 1, YY 1 transcription factor, YY-1, YY1 transcription factor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-YY1 Antibody [ARC0048] (A308157)

Western blot analysis of extracts of various cell lines, using Anti-YY1 Antibody [ARC0048] (A308157) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-YY1 Antibody [ARC0048] (A308157)

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Figure 3: Immunofluorescence - Anti-YY1 Antibody [ARC0048] (A308157)

Immunofluorescence analysis of C6 cells using Anti-YY1 Antibody [ARC0048] (A308157). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-YY1 Antibody [ARC0048] (A308157)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-YY1 Antibody [ARC0048] (A308157). This Western blot was performed on the immunoprecipitate using Anti-YY1 Antibody [ARC0048] (A308157) at a dilution of 1:2000.

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