Anti-YB1 Antibody (A15848)

Name: Anti-YB1 Antibody
Brand: Antibodies.com
SKU: A15848
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to YB1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-YB1 Antibody

Description

Rabbit polyclonal antibody to YB1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YB-1/YB-1/YBX1 (NP_004550.2).

Sequence

LRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNHYRRYPRRRGPPRNYQQNYQNSESGEKNEGSESAPEGQAQQRRPYRRRRFPPYYMRR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BP 8, CBF-A, CCAAT binding transcription factor I subunit A, CCAAT-binding transcription factor I subunit A, CSDA2, CSDB, DBPB, DNA binding protein B, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, MDR NF1, MGC104858, MGC110976, MGC117250, NSEP 1, NSEP1, Nuclease sensitive element binding protein 1, Nuclease-sensitive element-binding protein 1, p50, Q15905, Y-box binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB 1, YB-1, YBOX1_HUMAN, YBX 1, ybx1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-YB1 Antibody (A15848)

Western blot analysis of extracts of HepG2 cells, using Anti-YB1 Antibody (A15848) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-YB1 Antibody (A15848)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-YB1 Antibody (A15848) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-YB1 Antibody (A15848)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-YB1 Antibody (A15848) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-YB1 Antibody (A15848)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-YB1 Antibody (A15848) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.