Anti-YAP1 Antibody [ARC53477] (A308258)

Name: Anti-YAP1 Antibody [ARC53477]
Brand: Antibodies.com
SKU: A308258
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53477] antibody to YAP1 for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-YAP1 Antibody [ARC53477]

Description

Rabbit monoclonal [ARC53477] antibody to YAP1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:2,000-1:4,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).

Sequence

PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53477

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

65 kDa Yes associated protein, 65 kDa Yes-associated protein, COB1, YAP, YAp 1, YAP 65, YAP1_HUMAN, YAP2, YAP65, yes -associated protein delta, Yes associated protein 1, Yes associated protein 1 65kDa, Yes associated protein 2, yes associated protein beta, YKI, Yorkie homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (11)

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Figure 1: Western Blot - Anti-YAP1 Antibody [ARC53477] (A308258)

Western blot analysis of extracts of C6 cells, using Anti-YAP1 Antibody [ARC53477] (A308258) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-YAP1 Antibody [ARC53477] (A308258)

Western blot analysis of extracts of Mouse heart, using Anti-YAP1 Antibody [ARC53477] (A308258) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 3: Western Blot - Anti-YAP1 Antibody [ARC53477] (A308258)

Western blot analysis of extracts from wild type(WT) and YAP1 knockout (KO) HeLa cells, using Anti-YAP1 Antibody [ARC53477] (A308258) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 4: Immunohistochemistry - Anti-YAP1 Antibody [ARC53477] (A308258)

Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-YAP1 Antibody [ARC53477] (A308258) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-YAP1 Antibody [ARC53477] (A308258)

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Figure 6: Immunohistochemistry - Anti-YAP1 Antibody [ARC53477] (A308258)

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Figure 7: Immunohistochemistry - Anti-YAP1 Antibody [ARC53477] (A308258)

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Figure 8: Immunofluorescence - Anti-YAP1 Antibody [ARC53477] (A308258)

Immunofluorescence analysis of NIH/3T3 cells using Anti-YAP1 Antibody [ARC53477] (A308258) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 9: Immunofluorescence - Anti-YAP1 Antibody [ARC53477] (A308258)

Immunofluorescence analysis of U20S cells using Anti-YAP1 Antibody [ARC53477] (A308258) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 10: Immunofluorescence - Anti-YAP1 Antibody [ARC53477] (A308258)

Immunofluorescence analysis of HeLa cells using Anti-YAP1 Antibody [ARC53477] (A308258) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 11: Western Blot - Anti-YAP1 Antibody [ARC53477] (A308258)

Immunoprecipitation analysis of 300µg extracts of A-549 cells using 3µg of Anti-YAP1 Antibody [ARC53477] (A308258). This Western blot was performed on the immunoprecipitate using Anti-YAP1 Antibody [ARC53477] (A308258) at a dilution of 1:500.

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