XRCC6 Cell Based ELISA Kit (A103456)

XRCC6 Cell Based ELISA Kit

Cell-based (qualitative)

This XRCC6 Cell Based ELISA Kit allows for the detection of XRCC6 and the effects that certain stimulation conditions have on XRCC6 expression in different cell lines. Qualitative determination of XRCC6 concentration is achieved by an indirect ELISA format. In essence, the XRCC6 is captured by Anti-XRCC6 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this XRCC6 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, X-ray repair cross-complementing protein 6