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Anti-XPA Antibody [5F12] (A355)

This product is identical to Abcam (ab65963). Discover more for less.

Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution. Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions. Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.
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$535

Mouse monoclonal (5F12) antibody to XPA for WB, IF, ELISA and Inhibition Assay.

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$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-XPA Antibody [5F12]
Description
Mouse monoclonal (5F12) antibody to XPA.
Applications
WB, IF, ELISA, Inhibition Assay
Dilutions
WB: 1:1,000-1:10,000, IF: 1:100-1:1,000
Reactivity
Human
Immunogen
Recombinant full length human XPA protein.
Epitope
Amino acids 30-47.
Host
Mouse
Clonality
Monoclonal
Clone ID
5F12
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Ion exchange chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA repair protein complementing XP A cells, DNA repair protein complementing XP-A cells, DNA repair protein complementing XPA cells, Excision repair controlling, Xeroderma pigmentosum 1, Xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A complementing protein, Xeroderma pigmentosum group A-complementing protein, XP 1, XP1, Xpac, XPA_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution.Enlarge Image
Figure 1: Anti-XPA Antibody [5F12] (A355)
Anti-XPA Antibody
Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions.Enlarge Image
Figure 2: Anti-XPA Antibody [5F12] (A355)
Anti-XPA Antibody
Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.Enlarge Image
Figure 3: Immunofluorescence - Anti-XPA Antibody [5F12] (A355)
Anti-XPA Antibody
Publishing research using Anti-XPA Antibody [5F12] (A355)? Please let us know so that we can list the citation on this page.

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