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XIAP Cell Based ELISA Kit (A103267)

Western Blot - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.com Protocol Diagram - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.com Protocol Summary - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.com Western Blot - Control Antibody - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
XIAP Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This XIAP Cell Based ELISA Kit allows for the detection of XIAP and the effects that certain stimulation conditions have on XIAP expression in different cell lines. Qualitative determination of XIAP concentration is achieved by an indirect ELISA format. In essence, the XIAP is captured by Anti-XIAP Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this XIAP Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
AP 13, API3, Apoptosis Inhibitor 3, Baculoviral IAP repeat containing 4, Baculoviral IAP Repeat Containing Protein 4, Baculoviral IAP repeat-containing protein 4, BIRC 4, BIRC4, E3 ubiquitin-protein ligase XIAP, hIAP-3, hIAP3, HILP, IAP 3, IAP like protein, IAP-3, IAP-like protein, IAP3, ILP, ILP 1, ILP1, Inhibitor of apoptosis protein 3, Inhibitor of Apoptosis X Linked, Mammalian IAP Homologue A, MIHA, X linked IAP, X linked inhibitor of apoptosis, X linked inhibitor of apoptosis E3 ubiquitin protein ligase, X linked inhibitor of apoptosis protein, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP_HUMAN, XLP2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.comEnlarge Image
Figure 1: Western Blot - XIAP Cell Based ELISA Kit (A103267)
Western blot analysis of extracts from 293 cells, using Anti-XIAP (Ab-87) Antibody.
Protocol Diagram - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.comEnlarge Image
Figure 2: XIAP Cell Based ELISA Kit (A103267)
A diagram showing the protocol for XIAP Cell Based ELISA Kit.
Protocol Summary - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.comEnlarge Image
Figure 3: XIAP Cell Based ELISA Kit (A103267)
A summary of the protocol for XIAP Cell Based ELISA Kit.
Western Blot - Control Antibody - XIAP Cell Based ELISA Kit (CB5732) - Antibodies.comEnlarge Image
Figure 4: Western Blot - XIAP Cell Based ELISA Kit (A103267)
The mouse monoclonal antibody to GAPDH is used as a positive control inXIAP Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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