Anti-xCT Antibody (A84265)

Name: Anti-xCT Antibody
Brand: Antibodies.com
SKU: A84265
Price: 480.0 USD
Availability: InStock

Enlarge Image

$480

Goat polyclonal antibody to xCT for ELISA and IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-xCT Antibody

Description

Goat polyclonal antibody to xCT.

Applications

ELISA, IF

Dilutions

ELISA: 1:32,000, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human xCT.

Sequence

C-KGQTQNFKDAFSGRD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Amino acid transport system xc xCT, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, CCBR1, Cysteine/glutamate transporter, Cystine/glutamate transporter, OTTHUMP00000164578, SLC7A11, solute carrier family 7, Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11, Solute carrier family 7 member 11, Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN, XCT_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

Enlarge Image

Figure 1: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Enlarge Image

Figure 2: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Enlarge Image

Figure 3: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in Human Smooth Muscle (A) and Tonsil (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.1µg/ml and detected by chemiluminescence.

Enlarge Image

Figure 4: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in A549 (A) and U2OS (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.3µg/ml and detected by chemiluminescence.