Synthetic peptide corresponding to the internal region of human XBP1.
Sequence
C-NHSWEDTFANELFPQ
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
38 kDa
Predicted MW
40.1kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Tax responsive element binding protein 5, Tax-responsive element-binding protein 5, TREB5, X box binding protein 1, X box binding protein 2, X-box-binding protein 1, XBP 1, XBP-1, XBP1_HUMAN, XBP2
Figure 1: Western Blot - Anti-XBP1 Antibody (A83192)
XBP1 expression in HeLa nuclear lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-XBP1 Antibody (A83192) at 1µg/ml and detected by chemiluminescence.
XBP1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-XBP1 Antibody (A83192) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, ER, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
XBP1 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-XBP1 Antibody (A83192) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, ER, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
XBP1 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-XBP1 Antibody (A83192) at 5µg/ml and revealed with alkaline phosphatase (AP).
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