Anti-Wnt5a Antibody [ARC0405] (A308446)

Name: Anti-Wnt5a Antibody [ARC0405]
Brand: Antibodies.com
SKU: A308446
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0405] antibody to Wnt5a for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Wnt5a Antibody [ARC0405]

Description

Rabbit monoclonal [ARC0405] antibody to Wnt5a.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 281-380 of human WNT5A (P41221).

Sequence

RGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0405

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein Wnt-5a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Wnt5a Antibody [ARC0405] (A308446)

Western blot analysis of extracts of various cell lines, using Anti-Wnt5a Antibody [ARC0405] (A308446) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-Wnt5a Antibody [ARC0405] (A308446)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Wnt5a Antibody [ARC0405] (A308446) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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