Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hWNT 5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, Protein Wnt5a, Wingless type MMTV integration site family member 5A, Wnt 5a, WNT 5A protein, WNT 5A protein precursor, WNT5A protein precursor, WNT5A_HUMAN
Figure 1: Western Blot - Anti-Wnt5a Antibody (A89803)
Western blot analysis of extracts of various cell lines, using Anti-Wnt5a Antibody (A89803) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-Wnt5a Antibody (A89803) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Wnt5a Antibody (A89803) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Wnt5a Antibody (A89803) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of PC-12 cells using Anti-Wnt5a Antibody (A89803) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Western Blot - Anti-Wnt5a Antibody [ARC0405] (A308446) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308446_1.jpg?profile=product_alternative)
