Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
42.3 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hWNT 5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, Protein Wnt5a, Wingless type MMTV integration site family member 5A, Wnt 5a, WNT 5A protein, WNT 5A protein precursor, WNT5A protein precursor, WNT5A_HUMAN
Figure 1: Western Blot - Anti-Wnt5a Antibody (A304879)
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Anti-Wnt5a Antibody (A304879) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.
Immunohistochemistry analysis of human thyroid carcinoma, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Wnt5a Antibody (A304879) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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