Synthetic peptide corresponding to the internal region of human Wnt4.
Sequence
C-SNWLYLAKLSSVGS
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
40+35 kDa
Predicted MW
39.1kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
MGC123964, Protein Wnt-4, RP23-246F18.1, SERKAL, Wingless type MMTV integration site family member 4 precursor, WNT 4, WNT 4 protein precursor, WNT4_HUMAN
Figure 1: Western Blot - Anti-Wnt4 Antibody (A83822)
Wnt4 expression in Jurkat (A) and negative control U937 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Wnt4 Antibody (A83822) at 1µg/ml and detected by chemiluminescence.
Wnt4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Wnt4 Antibody (A83822) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
Wnt4 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Wnt4 Antibody (A83822) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
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