Synthetic peptide from the C-terminal of Human Wnt4 (aa. 250-351).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
33 kDa (isoform 2)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
MGC123964, Protein Wnt-4, RP23-246F18.1, SERKAL, Wingless type MMTV integration site family member 4 precursor, WNT 4, WNT 4 protein precursor, WNT4_HUMAN
Figure 1: Western Blot - Anti-Wnt4 Antibody (A304875)
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~39.1 kDa Wnt4 protein using Anti-Wnt4 Antibody (A304875) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~39.1 kDa. Other Band(s): 33 kDa.
Immunohistochemistry analysis of human kidney, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Wnt4 Antibody (A304875) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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