Anti-Wnt3a Antibody - BSA and Azide free (A283298)

$645

Rabbit polyclonal antibody to Wnt3a for WB, ELISA and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Wnt3a Antibody - BSA and Azide free
Description
Rabbit polyclonal antibody to Wnt3a.
Specificity
This antibody recognises human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways.
Applications
WB, ELISA, IHC-P
Dilutions
ELISA: 0.5 - 2 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human WNT-3a.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
1 mg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human WNT-3a antibody recognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways(Aulehla et al. 2003).
Synonyms
Protein Wnt 3a Precursor, Protein Wnt-3a, Wingless type MMTV integration site family member 3A, Wnt 3a, WNT3A protein, WNT3A_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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