Synthetic peptide corresponding to the internal region of human Wnt3.
Sequence
CGRGHNTRTEKRKEK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
40 kDa
Predicted MW
39.6kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Int 4, INT4, MGC131950, MGC138321, MGC138323, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, wingless type MMTV integration site family member 3, Wnt 3 proto oncogene protein, WNT 3 proto oncogene protein precursor, WNT3_HUMAN
Figure 1: Western Blot - Anti-Wnt3 Antibody (A84213)
Wnt3 expression in HEK293 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Wnt3 Antibody (A84213) at 1µg/ml and detected by chemiluminescence.
Wnt3 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Wnt3 Antibody (A84213) at 2µg/ml and revealed with horseradish peroxidase (HRP).
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