Synthetic peptide from the C-terminal of Human Proto-oncogene Wnt-3 (aa. 280-330).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Int 4, INT4, MGC131950, MGC138321, MGC138323, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, wingless type MMTV integration site family member 3, Wnt 3 proto oncogene protein, WNT 3 proto oncogene protein precursor, WNT3_HUMAN
Figure 1: Western Blot - Anti-Wnt3 Antibody (A304929)
Western blot analysis of mouse brain showing detection of 39.6 kDa WNT3 protein using Anti-Wnt3 Antibody (A304929) at 1:1,000 for 2 hours at room temperature with shaking. Load: 15 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: 39.6 kDa. Other Band(s): 50 kDa.
Immunohistochemistry analysis of human breast, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Wnt3 Antibody (A304929) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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