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Anti-VRK1 Antibody [5D1] (A418)

Western blot detection of VRK1 in the crude extracts of human cells. Lanes 1, 2, 3; HeLa cell extract (5x104 cells) with antibody dilutions at 1/100, 1/500, 1/1000. Lanes 4, 5, 6; U2OS cell extract (5x104 cells) with the antibody dilutions at 1/100, 1/500, 1/1,000. As secondary antibody, Alexa488 goat anti-mouse IgG was used. ImmunoStar?R LD (Wako, Tokyo) was used as chemiluminescence reagent and images were taken with BIO-RAD ChemiDocXRS Inhibition of VRK1 expression in human cells treated by RNAi. specific to VRK1. Immunoflorescence staining of VRK1 in HeLa cells, PA fixed.
$510

Mouse monoclonal (5D1) antibody to VRK1 for WB, IP, IF, IHC and ELISA.

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4-6 Business Days

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Name
Anti-VRK1 Antibody [5D1]
Description
Mouse monoclonal (5D1) antibody to VRK1.
Applications
WB, IP, IF, IHC, ELISA
Dilutions
WB: 1:200-1:1,000, IF: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the N-terminus of human VRK1 protein.
Sequence
MPRVKAAQAGRQSSAKRHL-C
Host
Mouse
Clonality
Monoclonal
Clone ID
5D1
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
MGC117401, MGC138280, MGC142070, PCH1, PCH1A, Serine/threonine protein kinase VRK1, Serine/threonine-protein kinase VRK1, Vaccinia related kinase 1, Vaccinia virus B1R related kinase 1, Vaccinia-related kinase 1, VRK1_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot detection of VRK1 in the crude extracts of human cells. Lanes 1, 2, 3; HeLa cell extract (5x104 cells) with antibody dilutions at 1/100, 1/500, 1/1000. Lanes 4, 5, 6; U2OS cell extract (5x104 cells) with the antibody dilutions at 1/100, 1/500, 1/1,000. As secondary antibody, Alexa488 goat anti-mouse IgG was used. ImmunoStar?R LD (Wako, Tokyo) was used as chemiluminescence reagent and images were taken with BIO-RAD ChemiDocXRSEnlarge Image
Figure 1: Anti-VRK1 Antibody [5D1] (A418)
Anti-VRK1 Antibody
Inhibition of VRK1 expression in human cells treated by RNAi. specific to VRK1.Enlarge Image
Figure 2: Anti-VRK1 Antibody [5D1] (A418)
Anti-VRK1 Antibody
Immunoflorescence staining of VRK1 in HeLa cells, PA fixed.Enlarge Image
Figure 3: Anti-VRK1 Antibody [5D1] (A418)
Anti-VRK1 Antibody
Publishing research using Anti-VRK1 Antibody [5D1] (A418)? Please let us know so that we can list the citation on this page.

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