Anti-VPS4a Antibody (A15539)

Name: Anti-VPS4a Antibody
Brand: Antibodies.com
SKU: A15539
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to VPS4a for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-VPS4a Antibody

Description

Rabbit polyclonal antibody to VPS4a.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human VPS4A (NP_037377.1).

Sequence

MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVME

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hVPS4, Protein SKD2, SKD1, SKD1 homolog, SKD1A, SKD2, Vacuolar protein sorting 4 homolog A, Vacuolar protein sorting 4, yeast, homolog of, A, Vacuolar protein sorting factor 4A, Vacuolar protein sorting-associated protein 4A, vacuolar sorting protein 4, VPS4, VPS4-1, VPS4A_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-VPS4a Antibody (A15539)

Western blot analysis of extracts of various cell lines, using Anti-VPS4a Antibody (A15539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-VPS4a Antibody (A15539)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-VPS4a Antibody (A15539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-VPS4a Antibody (A15539)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-VPS4a Antibody (A15539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-VPS4a Antibody (A15539)

Immunofluorescence analysis of NIH/3T3 cells using Anti-VPS4a Antibody (A15539) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).