Anti-VPS35 Antibody (A83699)

Name: Anti-VPS35 Antibody
Brand: Antibodies.com
SKU: A83699
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to VPS35 for ELISA, WB, IHC and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-VPS35 Antibody

Description

Goat polyclonal antibody to VPS35.

Applications

ELISA, WB, IHC, IF

Dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IHC: 8µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human VPS35.

Sequence

C-SPESEGPIYEGLIL

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

90 kDa

Predicted MW

91.7kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, Maternal-embryonic 3, MEM 3, MEM3, PARK17, TCCCTA00141, Vacuolar protein sorting 35, Vacuolar protein sorting 35 (yeast), Vacuolar protein sorting 35 homolog, Vacuolar protein sorting associated protein 35, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35, VPS 35, VPS35_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-VPS35 Antibody (A83699)

VPS35 expression in Human (A) Mouse (B) and Rat (C) Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-VPS35 Antibody (A83699) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-VPS35 Antibody (A83699)

VPS35 expression in HepG2 (A) and HEK293 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-VPS35 Antibody (A83699) at 0.03µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-VPS35 Antibody (A83699)

VPS35 expression in HEK293 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-VPS35 Antibody (A83699) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-VPS35 Antibody (A83699)

VPS35 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-VPS35 Antibody (A83699) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.