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Anti-VPS11 Antibody [ARC2325] (A306832)

Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.com Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.com Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.com Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2325] antibody to VPS11 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-VPS11 Antibody [ARC2325]
Description
Rabbit monoclonal [ARC2325] antibody to VPS11.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS11 (Q9H270).
Sequence
MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2325
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
108 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
END1, HGNC:14583, hVPS11, PEP5, PP3476, RING finger protein 108, RNF108, Vacuolar protein sorting 11 (yeast homolog), Vacuolar protein sorting 11 (yeast), Vacuolar protein sorting 11 homolog, Vacuolar protein sorting 11 homolog (S. cerevisiae), Vacuolar protein sorting associated protein 11 homolog, Vacuolar protein sorting protein 11, Vacuolar protein sorting-associated protein 11 homolog, VPS11_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832)
Western blot analysis of extracts of various cell lines, using Anti-VPS11 Antibody [ARC2325] (A306832) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-VPS11 Antibody [ARC2325] (A306832)
Western blot analysis of extracts of various cell lines, using Anti-VPS11 Antibody [ARC2325] (A306832) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-VPS11 Antibody [ARC2325] (A306832) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-VPS11 Antibody [ARC2325] (A306832)
Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-VPS11 Antibody [ARC2325] (A306832) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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