Anti-Vitamin D Receptor Antibody (A83736)

Name: Anti-Vitamin D Receptor Antibody
Brand: Antibodies.com
SKU: A83736
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Vitamin D Receptor for ELISA, WB and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Vitamin D Receptor Antibody

Description

Goat polyclonal antibody to Vitamin D Receptor.

Applications

ELISA, WB, Flow Cytometry

Dilutions

ELISA: 1:16,000, WB: 2-3µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human Vitamin D Receptor.

Sequence

CGNQDYKYRVSD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

55 kDa

Predicted MW

55.2kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

1, 1 25 dihydroxyvitamin D3 receptor, 1,25 dihydroxyvitamin D3 receptor, 1,25-@dihydroxyvitamin D3 receptor, 25-dihydroxyvitamin D3 receptor, Member 1, NR1I1, Nuclear receptor subfamily 1 group I member 1, PPP1R163, Protein phosphatase 1, regulatory subunit 163, VDR, VDR_HUMAN, Vitamin D (1,25- dihydroxyvitamin D3) receptor, Vitamin D hormone receptor, Vitamin D nuclear receptor variant 1, Vitamin D3 receptor

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Vitamin D Receptor Antibody (A83736)

Vitamin D Receptor expression in U937 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Vitamin D Receptor Antibody (A83736) at 3µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-Vitamin D Receptor Antibody (A83736)

Vitamin D Receptor expression in Human Placenta (A) and Duodenum (B) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Vitamin D Receptor Antibody (A83736) at 3µg/ml (A) or 2µg/ml (B) and detected by chemiluminescence.

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Figure 3: Flow Cytometry - Anti-Vitamin D Receptor Antibody (A83736)

Vitamin D Receptor expression in U937 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Vitamin D Receptor Antibody (A83736) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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