Anti-Vimentin Antibody (A85420)

Immunofluorescence analysis of HeLa cells co-stained with Anti-Vimentin Antibody (1:5,000 | green) and Anti-Actin Antibody (A85388 | 1:500 | red). Blue is DAPI staining of nuclear DNA. The Anti-Vimentin Antibody stains the 10nm or intermediate filament network of the cytoskeleton. The Anti-Actin Antibody labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

View of HeLa cells stained with Anti-Vimentin Antibody (red) and Anti-Lamin A/C Antibody (A85444 | green). DNA is blue. Anti-Vimentin Antibody reveals strong cytoplasmic intermediate filament staining, while Anti-Lamin A/C Antibody reveals strong nuclear lamina staining.

Western blot analysis of whole cell lysates using Anti-Vimentin Antibody (1:5000 | green): [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH 3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of 54 kDa.

Western blot of crude extract of HeLa cells stained with Anti-Vimentin Antibody - showing a single strong clean band at 55 kDa.