Recombinant rabbit monoclonal [VIL1/8105R] antibody to Villin.
Specificity
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Applications
IHC-P
Positive Control
A549, HepG2 or HCT116 cells. Human colon or rectum.
Dilutions
IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment (around Amino Acids 600-700) of human Villin protein. The exact sequence is proprietary.
Host
Rabbit
Clonality
Monoclonal
Clone ID
VIL1/8105R
Isotype
IgG
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
93 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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![Immunohistochemistry - Anti-Villin Antibody [VIL1/4107R] (A250302) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250302_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Villin Antibody [VIL1/4107R] - BSA and Azide free (A253482) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253482_1.jpg?profile=product_alternative)