Anti-Versican Antibody [ARC2216] (A308316)

Name: Anti-Versican Antibody [ARC2216]
Brand: Antibodies.com
SKU: A308316
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2216] antibody to Versican for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Versican Antibody [ARC2216]

Description

Rabbit monoclonal [ARC2216] antibody to Versican.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611).

Sequence

QGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2216

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

265 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chondroitin sulfate proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan core protein, cartilage, CSPG2, CSPG2_HUMAN, ERVR, GHAP, Glial hyaluronate binding protein, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, PGM, VCAN, Versican core protein, Versican proteoglycan, WGN, WGN 1, WGN1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Versican Antibody [ARC2216] (A308316)

Western blot analysis of extracts of various cell lines, using Anti-Versican Antibody [ARC2216] (A308316) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Versican Antibody [ARC2216] (A308316)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Versican Antibody [ARC2216] (A308316) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Versican Antibody [ARC2216] (A308316)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Versican Antibody [ARC2216] (A308316) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Versican Antibody [ARC2216] (A308316)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Versican Antibody [ARC2216] (A308316) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.