Recombinant Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321)

Name: Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free
Brand: Antibodies.com
SKU: A323321
Price: 295.0 USD
Availability: InStock

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$295

Recombinant human monoclonal antibody to VEGFA for ELISA, FACS, Functional Assay and In Vivo.

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Delivery

Freight Charges

$40 to United States

Lead Time

7-10 Business Days

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+1 (314) 370-6046

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Recombinant Antibody

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Specifications

Name

Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free

Description

Recombinant human monoclonal antibody to VEGFA.

Applications

ELISA, FACS, Functional Assay, In Vivo

Reactivity

Human, Cynomolgus Macaque, Mouse

Host

Human

Clonality

Monoclonal

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A affinity chromatography.

Concentration

Reconstitution dependent.

Molecular Weight

This antibody has a predicted MW of 146.5 kDa.

Purity

> 95% (by SDS-PAGE and SEC-HPLC).

Product Form

Lyophilized

Reconstitution

Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!

Formulation

Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, MGC70609, MVCD1, vascular endothelial growth factor, Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, Vascular permeability factor, Vegf, VEGF A, VEGF-A, VEGF120 , VEGFA_HUMAN, VPF

Suitable Secondaries

See all Anti-Human IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321)

SDS-PAGE analysis of Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.

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Figure 2: Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321)

SEC-HPLC analysis of Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321) confirms that the purity of this antibody is 97.8%.

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Figure 3: Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321)

Immobilized recombinant human VEGF165 protein (His tag) at 2 µg/ml is bound by Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321). EC₅₀ = 0.003018 µg/ml.

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Figure 4: Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321)

Anti-VEGFA Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323321) inhibited the tumor growth of COLO205 on BALB/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 42.0% at 0.95 mpk at D31.

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