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Anti-VEGFA Antibody (A89855)

Western Blot - Anti-VEGFA Antibody (A89855) - Antibodies.com Immunofluorescence - Anti-VEGFA Antibody (A89855) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to VEGFA for WB and ICC/IF.

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5-8 Business Days

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Name
Anti-VEGFA Antibody
Description
Rabbit polyclonal antibody to VEGFA.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-126AA of human VEGFA (NP_001165099.1).
Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
23 kDa / 27 kDa / 45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, MGC70609, MVCD1, vascular endothelial growth factor, Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, Vascular permeability factor, Vegf, VEGF A, VEGF-A, VEGF120 , VEGFA_HUMAN, VPF
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-VEGFA Antibody (A89855) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-VEGFA Antibody (A89855)
Western blot analysis of extracts of various cell lines, using Anti-VEGFA Antibody (A89855) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-VEGFA Antibody (A89855) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-VEGFA Antibody (A89855)
Immunofluorescence analysis of NIH/3T3 cells using Anti-VEGFA Antibody (A89855) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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