Anti-VEGFA Antibody (A14925)

Name: Anti-VEGFA Antibody
Brand: Antibodies.com
SKU: A14925
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to VEGFA for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-VEGFA Antibody

Description

Rabbit polyclonal antibody to VEGFA.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 68-147 of human VEGFA (NP_001165099.1).

Sequence

EIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa / 22 kDa / 27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, MGC70609, MVCD1, vascular endothelial growth factor, Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, Vascular permeability factor, Vegf, VEGF A, VEGF-A, VEGF120 , VEGFA_HUMAN, VPF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-VEGFA Antibody (A14925)

Western blot analysis of extracts of various cell lines, using Anti-VEGFA Antibody (A14925) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-VEGFA Antibody (A14925)

Immunofluorescence analysis of A431 cells using Anti-VEGFA Antibody (A14925) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-VEGFA Antibody (A14925)

Immunofluorescence analysis of NIH/3T3 cells using Anti-VEGFA Antibody (A14925) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-VEGFA Antibody (A14925)

Immunofluorescence analysis of PC-12 cells using Anti-VEGFA Antibody (A14925) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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