Western Blot - Anti-VEGF Receptor 2 (phospho Tyr1175) Antibody (A306370)
Western blot analysis of extracts of various cell lines, using Anti-VEGF Receptor 2 (phospho Tyr1175) Antibody (A306370) at 1:1,000 dilution. HUVEC cells were treated by VEGF (100 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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