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VE-Cadherin Cell Based ELISA Kit (A103371)

Western Blot - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.com Protocol Diagram - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.com Protocol Summary - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.com Western Blot - Control Antibody - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
VE-Cadherin Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This VE-Cadherin Cell Based ELISA Kit allows for the detection of VE-Cadherin and the effects that certain stimulation conditions have on VE-Cadherin expression in different cell lines. Qualitative determination of VE-Cadherin concentration is achieved by an indirect ELISA format. In essence, the VE-Cadherin is captured by Anti-VE-Cadherin Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this VE-Cadherin Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
7B 4, 7B4, 7B4 antigen, CADH5_HUMAN, Cadherin 5, Cadherin 5 type 2, Cadherin 5, type 2 (vascular endothelium), Cadherin 5, type 2, VE cadherin (vascular epithelium), cadherin, vascular endothelial, cadherin, vascular endothelial, 1, Cadherin-5, Cadherin5, CD 144, CD144, CD144 antigen, CDH 5, CDH5, CDH5 protein, Endothelial specific cadherin, FLJ17376, OTTHUMP00000174777, Vascular endothelial cadherin, Vascular epithelium cadherin, VE Cad, VE Cadherin, VEC
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comEnlarge Image
Figure 1: Western Blot - VE-Cadherin Cell Based ELISA Kit (A103371)
Western blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3mM 40', using Anti-VE-Cadherin (phopho Tyr731) Antibody.
Protocol Diagram - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comEnlarge Image
Figure 2: VE-Cadherin Cell Based ELISA Kit (A103371)
A diagram showing the protocol for VE-Cadherin Cell Based ELISA Kit.
Protocol Summary - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comEnlarge Image
Figure 3: VE-Cadherin Cell Based ELISA Kit (A103371)
A summary of the protocol for VE-Cadherin Cell Based ELISA Kit.
Western Blot - Control Antibody - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comEnlarge Image
Figure 4: Western Blot - VE-Cadherin Cell Based ELISA Kit (A103371)
The mouse monoclonal antibody to GAPDH is used as a positive control inVE-Cadherin Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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