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Anti-VCAM1 Antibody (A12598)

Western Blot - Anti-VCAM1 Antibody (A12598) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to VCAM1 for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-VCAM1 Antibody
Description
Rabbit polyclonal antibody to VCAM1.
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
Sequence
NRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVEL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
95 - 120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD106, CD106 Antigen, INCAM 100 , INCAM-100, L1CAM, MGC99561, V-CAM 1, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, VCAM1_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-VCAM1 Antibody (A12598) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-VCAM1 Antibody (A12598)
Western blot analysis of extracts of HUVEC cells, using Anti-VCAM1 Antibody (A12598) at 1:500 dilution. HUVEC cells were treated by Human Tumor Necrosis Factor-alpha (hTNF-alpha) (10 ng/ml) for 16 hoursThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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