Rat monoclonal [429 (MVCAM.A)] antibody to VCAM1 (PE).
Specificity
This antibody, also known as clone MVCAM.A, recognizes an extracellular epitope of murine CD106, a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a co-stimulation molecule.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Mouse
Immunogen
Murine preadipose cell line PA6.
Host
Rat
Clonality
Monoclonal
Clone ID
429 (MVCAM.A)
Isotype
IgG2a
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Predicted MW
100-110 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of murine bone marrow cell suspension stained with Anti-CD106 Antibody [429 (MVCAM.A)] (PE). Concentration in sample 0.56µg/ml.
Separation of murine CD160 positive cells (red-filled) from murine CD160 negative myeloid cells (black-dashed) in flow cytometry analysis of murine bone marrow cell suspension using Anti-CD106 Antibody [429 (MVCAM.A)] (PE). Concentration in sample is 0.56µg/ml.
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