Mouse monoclonal [1.G11B1] antibody to VCAM1 (FITC).
Specificity
This antibody recognises human VCAM-1, a ~110 kDa molecule whose ligand is VLA4. The antigen is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts. Mouse anti Human CD106 antibody, clone 1.G11B1 inhibits cellular adhesion mediated by VCAM-1 (Patel 1998).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Pig, Rhesus Monkey
Host
Mouse
Clonality
Monoclonal
Clone ID
1.G11B1
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD106 antibody, clone 1.G11B1 recognizes human VCAM-1, a ~110 kDa molecule whose ligand is VLA4. The antigen is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts.Mouse anti Human CD106 antibody, clone 1.G11B1 inhibits cellular adhesion mediated by VCAM-1 (Patel 1998).