Rabbit polyclonal antibody to VAV1 (phospho Tyr174).
Specificity
This antibody recognises VAV1, also known as vav guanine nucleotide exchange factor 1 when phosphorylated at tyrosine 174. VAV1 is a member of the Vav family of guanine nucleotide exchange factors (GEFs). GEFs are involved in the activation of small GTPases which play a role in many signaling pathways. Potential substrates for VAV1 activity include Rac1, Cdc42 RhoA and RhoG. VAV1 is phosphorylated on tyrosine 174 leads to conformational changes and activation of VAV1 (Katzav 2015).
Applications
WB
Dilutions
WB: 1:500 - 1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
Phospho-specific peptide corresponding to residues surrounding Tyr174 of human VAV1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti VAV1 (pTyr174) antibody recognizes VAV1, also known as vav guanine nucleotide exchange factor 1 when phosphorylated at tyrosine 174. VAV1 is a member of the Vav family of guanine nucleotide exchange factors (GEFs). GEFs are involved in the activation of small GTPases which play a role in many signaling pathways. Potential substrates for VAV1 activity include Rac1, Cdc42 RhoA and RhoG. VAV1 is phosphorylated on tyrosine 174 leads to conformational changes and activation of VAV1 (Katzav 2015).
