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Anti-VAT1 Antibody (A91769)

Western Blot - Anti-VAT1 Antibody (A91769) - Antibodies.com Western Blot - Anti-VAT1 Antibody (A91769) - Antibodies.com Immunofluorescence - Anti-VAT1 Antibody (A91769) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to VAT1 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-VAT1 Antibody
Description
Rabbit polyclonal antibody to VAT1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human VAT1 (NP_006364.2).
Sequence
GVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ20230, Membrane protein of cholinergic synaptic vesicles, MGC124668, OTTMUSP00000002784, RP23-328K2.5, Synaptic vesicle membrane protein VAT 1 homolog, Synaptic vesicle membrane protein VAT-1 homolog, VAT 1, VAT1_HUMAN, VATI, Vesicle amine transport protein 1, Vesicle amine transport protein 1 homolog (T. californica)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-VAT1 Antibody (A91769) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-VAT1 Antibody (A91769)
Western blot analysis of extracts of various cell lines, using Anti-VAT1 Antibody (A91769) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5min.
Western Blot - Anti-VAT1 Antibody (A91769) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-VAT1 Antibody (A91769)
Western blot analysis of extracts from normal (control) and VAT1 knockout (KO) 293T cells, using Anti-VAT1 Antibody (A91769) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-VAT1 Antibody (A91769) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-VAT1 Antibody (A91769)
Immunofluorescence analysis of L929 cells using Anti-VAT1 Antibody (A91769) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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