Anti-V5-Tag Antibody (A50030)

All lanes: V5-tag mouse monoclonal antibody HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1: 2000 dilution (blue DAPI, red anti-V5)

IP antibody use：5ug V5 Mouse IgG1 per ml 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with V5-tag fusion protein3) IP (untransfected 293+anti-V5 mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5) IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6) IP (transfected 293+Protein G) 7) Recombinant protein (E.coli)