Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein, using Anti-V5 Tag Antibody (A306052) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
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