Anti-USP5 Antibody (A14537)

Name: Anti-USP5 Antibody
Brand: Antibodies.com
SKU: A14537
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to USP5 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-USP5 Antibody

Description

Rabbit polyclonal antibody to USP5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).

Sequence

MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, ISOT 1, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin isopeptidase T, Ubiquitin specific peptidase 5, Ubiquitin specific peptidase 5 (isopeptidase T), Ubiquitin specific processing protease 5, Ubiquitin specific protease 5 (isopeptidase T), Ubiquitin specific protease 5 (ubiquitin isopeptidase T), Ubiquitin thioesterase 5, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, UBP5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-USP5 Antibody (A14537)

Western blot analysis of extracts of various cell lines, using Anti-USP5 Antibody (A14537) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-USP5 Antibody (A14537)

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-USP5 Antibody (A14537) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-USP5 Antibody (A14537)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-USP5 Antibody (A14537) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-USP5 Antibody (A14537)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-USP5 Antibody (A14537) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.