Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Mitochondrial uncoupling protein 3, SLC25A9, Solute carrier family 25 member 9, UCP 3, UCP3_HUMAN, Uncoupling protein 3, Uncoupling protein 3 mitochondrial proton carrier
Figure 1: Western Blot - Anti-UCP3 Antibody (A91768)
Western blot analysis of extracts of various cell lines, using Anti-UCP3 Antibody (A91768) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-UCP3 Antibody (A91768) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using Anti-UCP3 Antibody (A91768) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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