Synthetic peptide corresponding to the internal region of human UCP2.
Sequence
C-DSVKQFYTKGSEH
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
37 kDa
Predicted MW
33.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BMIQ4, Mitochondrial uncoupling protein 2, SLC25A8, Solute carrier family 25 member 8, UCP 2, UCP2_HUMAN, UCPH, Uncoupling protein 2, Uncoupling protein 2 mitochondrial proton carrier
Figure 1: Western Blot - Anti-UCP2 Antibody (A84323)
UCP2 expression in Rat Adipose (A) and Mouse Spleen (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-UCP2 Antibody (A84323) at 1µg/ml and detected by chemiluminescence.
UCP2 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-UCP2 Antibody (A84323) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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