Anti-UCP1 Antibody (A15019)

Name: Anti-UCP1 Antibody
Brand: Antibodies.com
SKU: A15019
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to UCP1 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Highlighted products for Anti-UCP1 Antibody

A84145

Anti-UCP1 Antibody

Goat polyclonal antibody to UCP1 for ELISA and WB.

A84145

Anti-UCP1 Antibody

Goat polyclonal antibody to UCP1 for ELISA and WB.

Specifications

Name

Anti-UCP1 Antibody

Description

Rabbit polyclonal antibody to UCP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).

Sequence

QEFLTAGKETAPSLGSKILAGLTTGGVAVFIGQPTEVVKVRLQAQSHLHGIKPRYTGTYNAYRIIATTEGLTGLWKGTTPNLMRSVIINCTELVTYDLMKE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

mitochondrial brown fat uncoupling protein, Mitochondrial brown fat uncoupling protein 1, SLC25A7, Solute carrier family 25 member 7, Thermogenin, UCP, UCP 1, UCP1_HUMAN, Uncoupling protein 1, uncoupling protein 1 (mitochondrial, proton carrier)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-UCP1 Antibody (A15019)

Western blot analysis of Mouse brown adipose tissue, using Anti-UCP1 Antibody (A15019) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-UCP1 Antibody (A15019)

Western blot analysis of Rat brown adipose tissue, using Anti-UCP1 Antibody (A15019) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-UCP1 Antibody (A15019)

Immunohistochemistry analysis of paraffin-embedded rat brown adipose using Anti-UCP1 Antibody (A15019) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-UCP1 Antibody (A15019)

Immunofluorescence analysis of rat brown adipose cells using Anti-UCP1 Antibody (A15019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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