Anti-UCHL3 Antibody [ARC1874] (A307232)

Name: Anti-UCHL3 Antibody [ARC1874]
Brand: Antibodies.com
SKU: A307232
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1874] antibody to UCHL3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-UCHL3 Antibody [ARC1874]

Description

Rabbit monoclonal [ARC1874] antibody to UCHL3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UCHL3 (P15374).

Sequence

MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1874

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCH-L3, UCHL3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-UCHL3 Antibody [ARC1874] (A307232)

Western blot analysis of extracts of various cell lines, using Anti-UCHL3 Antibody [ARC1874] (A307232) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-UCHL3 Antibody [ARC1874] (A307232)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-UCHL3 Antibody [ARC1874] (A307232) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-UCHL3 Antibody [ARC1874] (A307232)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-UCHL3 Antibody [ARC1874] (A307232) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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