Rabbit polyclonal antibody to UBF (Phospho-Ser484)
Specificity
The antibody detects endogenous levels of UBF only when phosphorylated at serine 484.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HeLa cells, treated with calyculin A (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody #12154. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody #12154. The picture on the right is treated with the synthesized peptide.
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