Anti-UBE2C Antibody (A83441)

Name: Anti-UBE2C Antibody
Brand: Antibodies.com
SKU: A83441
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to UBE2C for ELISA, WB, IF, Flow Cytometry and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-UBE2C Antibody

Description

Goat polyclonal antibody to UBE2C.

Applications

ELISA, WB, IF, Flow Cytometry, IP

Dilutions

ELISA: 1:32,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human UBE2C.

Sequence

C-QETYSKQVTSQEP

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

19 kDa

Predicted MW

19.7kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cyclin selective ubiquitin carrier protein, dJ447F3.2, Mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH 10, UbcH10, UBE 2C, UBE2C_HUMAN, Ubiquitin carrier protein C, Ubiquitin carrier protein E2 C, Ubiquitin carrier protein E2C, Ubiquitin conjugating enzyme E2 C, Ubiquitin conjugating enzyme E2C, Ubiquitin protein ligase C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-UBE2C Antibody (A83441)

UBE2C expression in HEK293 (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-UBE2C Antibody (A83441) at 2µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-UBE2C Antibody (A83441)

UBE2C expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic/plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 3: Immunofluorescence - Anti-UBE2C Antibody (A83441)

UBE2C expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 4: Flow Cytometry - Anti-UBE2C Antibody (A83441)

UBE2C expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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