Anti-U2AF65 Antibody [ARC1029] (A305827)

Name: Anti-U2AF65 Antibody [ARC1029]
Brand: Antibodies.com
SKU: A305827
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1029] antibody to U2AF65 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-U2AF65 Antibody [ARC1029]

Description

Rabbit monoclonal [ARC1029] antibody to U2AF65.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2/U2AF2/U2AF65 (P26368).

Sequence

EVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1029

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hU2AF(65), hU2AF65, Splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2 snRNP auxiliary factor large subunit, U2af2, U2AF2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-U2AF65 Antibody [ARC1029] (A305827)

Western blot analysis of extracts of various cell lines, using Anti-U2AF65 Antibody [ARC1029] (A305827) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-U2AF65 Antibody [ARC1029] (A305827)

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Figure 3: Immunohistochemistry - Anti-U2AF65 Antibody [ARC1029] (A305827)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-U2AF65 Antibody [ARC1029] (A305827) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-U2AF65 Antibody [ARC1029] (A305827)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-U2AF65 Antibody [ARC1029] (A305827). This Western blot was performed on the immunoprecipitate using Anti-U2AF65 Antibody [ARC1029] (A305827) at a dilution of 1:1000.

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