Sheep polyclonal antibody to Tyrosine Hydroxylase.
Specificity
This antibody recognises rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
Applications
WB, IHC-Fr, IF
Dilutions
IF: 1:1,000, IHC-Fr: 1:1,000, WB: 1:1,000
Reactivity
Rat, Mammals, Rabbit
Immunogen
Rat Tyrosine hydroxylase purified from pheochromocytoma.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Molecular Weight
Approximately 60 kDa in rat caudate cell lysates.
Product Form
Liquid
Formulation
Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Rat tyrosine hydroxylase antibody recognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
